|
 Home > Diagnostic Services > Classical MAP/RAP test
|
The MAP test is a technique used to detect the presence of murine infectious agents in biologic preparations, generally cell lines, tumors and monoclonal antibodies. It is based on the premises that any viable infectious agents inoculated into a murine host will replicate and stimulate a detectable antibody response.
Classical MAP testing for the full complement of murine viruses involves inoculation of weanling mice with the sample and collection of blood 28 days later at the Central Animal Laboratory of the University Medical Center Nijmegen. The animals are housed in IVCs (individually ventilated cages) or isolators. Serologic tests are subsequently performed after the prescribed exposure period.
Three or 4 days after inoculation a first blood sample is taken and tested for elevated levels of LDH (lactate dehydrogenase) in plasma which is an indication for LDV infection.
Analogous to the MAP test, QM Diagnostics can detect rat infectious agents using a Rat Antibody Production (RAP) test or hamster infectious agents (HAP).
QM Diagnostics offers a validated panel of PCR's (conventional and real time PCR) as an alternative to the classical MAP/RAP or HAP test, see the page MAP/RAP PCR test.
All diagnostic tests in the table are covered by the CCKL accreditation.
| Classical Antibody Production tests |
MAP test (17 agents) |
RAP test (11 agents) |
HAP test (5 agents) |
| Ectromelia virus | x | ||
| K virus (K) | x | ||
| Lactic dehydrogenase virus (LDV) | x | ||
| Lymphocytic choriomeningitis virus (LCMV) | x | x | |
| Minute virus of mice (MVM) | x | ||
| Mouse adenovirus (MadV), FL type 1 | x | x | |
| Mouse adenovirus (MadV), K87 | x | x | |
| Mouse cytomegalovirus (MCMV) | x | ||
| Mouse hepatitis virus (MHV) | x | ||
| Mouse parvo virus (MPV) | x | ||
| Mouse polyoma virus | x | ||
| Mouse rotavirus (EDIM) | x | ||
| Mouse thymic virus (MTV) | x | ||
| Pneumonia virus of mice (PVM) | x | x | x |
| Reovirus 3 (Reo 3) | x | x | x |
| Sendai virus | x | x | x |
| Theiler's encephalomyelitis virus (TMEV) | x | x | |
| Hantavirus | x | ||
| Kilham rat virus (KRV) | x | ||
| Rat coronavirus / Sialoacryoadenitis virus (RCV / SDA) | x | ||
| Rat parvo virus (RPV) | x | ||
| Toolan (H-1) | x | ||
| Simian virus 5 (SV5) | x |